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Email: info@polysciences.de
TECHNICAL DATA SHEET 741
Page 1 of 6
Microring
®
Bacterial Detectors
Polysciences offers a wide variety of products specific for microbiological and bacterial detection applications. Microring
®
bacterial
detectors are simple, rapid test methods which utilize specific growth factors to identify different microorganisms.
Microring
®
XV
Cat. #24644
(50 rings/pkg)
Microring
®
XV is a paper ring used in Haemophilus identification. It comes complete with individual printed tips containing haemin
(X-factor) and nicotinamide adenine dinucleotide-NAD (V-factor) or X+V factor. This ring differentiates Haemophilus species on the
basis of growth requirements for X and V factors and can be identified by their growth patterns close to the tips. V-tip also contains
δ
-aminolaevulinic acid (ALA) for Porphyrin test. (The porphyrin test is best performed at the start when the ring is laid on the plate
prior to incubation. Using a loop take a sample of the test organism from a growing culture and spread lightly over the V-tip.
Non-haemin requiring species will cause an orange-red glow to be seen from the V-tip.)
Procedure
1. Spread sample of the test organism onto an agar plate of Tryptone Soy Agar or Columbia Agar (without blood).
2. Using sterile forceps, carefully place Microring
®
XV in the center of the plate. Press each tip gently to ensure contact with surface.
3. Incubate plate overnight at 37° C.
4. Following incubation, growth patterns around each tip are read and used to identify the organism.
Differentiation of Haemophilus species
Species
H. influenzae
H. aegypticus
H. parainfluenzae
H. haemolyticus
H. parahaemolyticus
Growth results at tip
XV
V
X
NG NG
G
NG NG
G
G
NG G
NG
NG
NG
G
G
G
Growth factor
requirement
XV
XV
V
XV
V
XV = Haemin and NAD
Porphyrin
synthesis (ALA)
-
-
+
-
+
NG = No growth
Haemolysis on
horse blood*
-
-
-
+
+
G = Zone of growth
X = Haemin V = NAD and
δ
-aminolaevulinic acid (ALA)
* Haemolysis recorded from primary culture.
Microring
®
XV
continues - pg 2
Should any of our materials fail to perform to our specifications, we will be pleased to provide replacements or return the purchase price. We solicit your inquiries concerning all needs for life sciences work. The information
given in this bulletin is to the best of our knowledge accurate, but no warranty is expressed or implied. It is the user’s responsibility to determine the suitability for their own use of the products described herein, and since
conditions of use are beyond our control, we disclaim all liability with respect to the use of any material supplied by us. Nothing contained herein shall be construed as a recommendation to use any product or to practice
any process in violation of any law or any government regulation.
© Polysciences, Inc.
Active: Jan/2007
Data Sheet #741
1
GEC [ GENERAL ELECTRIC COMPANY ]
